Analysis Note

ControlRat brain cytosol tissue lysate

Application

Research Sub CategorySignaling Neuroscience

Research CategoryNeuroscience

Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated GRIP-1 from rat brain cytosol tissue lysate.

Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected GRIP-1 in rat brain cells.

Detect GRIP-1 using this Anti-GRIP-1 Antibody, C-terminus validated for use in WB, IP, IH(P).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Glutamate receptor interacting protein-1 (GRIP-1) is a multi-PDZ domain scaffold protein and a member of the steroid receptor coactivator (SRC) family of proteins. GRIP-1 interacts and maneuvers kinesin heavy chains to dendrites by acting as the power source for AMPA receptors. During initial stages of embryonic development, GRIP-1 is essential for typical cell-matrix interactions.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of rat GRIP-1.

Linkage

Replaces: 06-986

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in rat brain cytosol tissue lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected GRIP-1 on 10 µg of rat brain cytosol tissue lysate.

Specificity

This antibody recognizes GRIP-1 at the C-terminus.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~121 kDa observed